Avaliação de genes responsivos ao estresse salino no Musgo Bryum Argenteum Hedw

Abstract

Salt stress considerably affects plant growth and development. Mosses, the predominant vegetation in island environments, can withstand high levels of salinity. To better understand the salinity tolerance mechanisms of Bryum argenteum moss, RNA-seq techniques were applied to analyze transcriptomic alterations in samples submitted to different concentrations of NaCl (200 mM, 400 mM, 600 mM and 800 mM) for 6 hours and 24h compared to the control group. In addition to the establishment of in vitro cultivation and genome sequencing. We found that higher concentrations of hypochlorite, when combined with a short immersion time, disinfect B. argenteum without causing damage to the plant, just as the MS culture medium provides greater development of protonemas and KNOP, in turn, of gametophytes. Compared to genomes of mosses considered as complete, our genome has greater similarities with the genomes of Ceratodon purpureus and Pohlia nutans, with the gap between the genomes of Bryum argenteum and Physcomitrium patens being much more representative than in the other genomes compared with our assembly. As for the analysis of genetic ontology, there were GOs related to the metabolic process of carbohydrates (GO:0005975); molecular function with oxidoreductase activity (GO:0016491); integral membrane component (GO:0016021); chloroplast thylakoid membrane composition (GO:0009535); photosystem II (GO:0009523); chloroplast (GO:0009507); among others, with emphasis on the photosynthetic part that undergoes changes as NaCl levels are high. The 6h treatment had more contrasting than similar genes when compared to the 24h treatment, which shows how quickly the plant generates a salt response in order to protect itself against stress, thus, a slow and gradual adaptation occurs through molecular responses to stresses, which leads to acclimatization. Finally, we conclude that the same sets of genes are involved in the reaction of plants to osmoregulation and/or in responses to ionic changes, showing that these mechanisms are also conserved in many other terrestrial plants even if they are not under the influence of marine spraying.