Avaliação das alterações comportamentais e bioquímicas induzidas por uma dieta hiperglicídica em drosophila melanogaster

Abstract

The objective of this study was to evaluate the behavioral and biochemical changes induced by a hyperglycemic diet in Drosophila melanogaster. Adult female flies up to 3 days old were divided into four groups of 50 flies each: (1) standard diet, (2) hyperglycemia diet 10%, (3) hyperglycemia diet 30%, (4) diet Hyperglycemia 40%. The flies were exposed to their respective diets for 10 days. For the hyperglycemic diets, sucrose was added in the standard diet at concentrations of 10, 30 and 40%. Mortality, body weight at the beginning and at the end of the experiment were evaluated in order to verify the weight gain and at the end of the 10 days the locomotor activity was evaluated using the negative geotaxia test and the open field test. At the end of the experiment the flies were cryoanestesized, euthanized and homogenized for glucose and triglyceride analyzes. Our results demonstrate that the ingestion of the hyperglycemic diet culminates in weight gain as well as elevation of glucose and triglyceride levels in Drosophila melanogaster at concentrations of 30% and 40% of sucrose. The hyperglycemic diet had a negative impact on their locomotor behavior, reduced mobility, as well as an increase in climbing time and a reduction in the stimulus to flight. This work evidences that the hyperglycemic diet caused behavioral and biochemical changes in Drosophila melanogaster, being thus a good model for the study of metabolic alterations such as type 2 diabetes mellitus.