Atividade antimicrobiana in vitro e in vivo do extrato etanólico de própolis contra as principais bactérias envolvidas na mastite bovina

Abstract

The objective of this study was to evaluate the antimicrobial activity of ethanol extract of propolis (EEP), in vitro and in vivo. For in vitro analysis, we tested the action of EEP against the main bacterial agents causing mastitis and this was followed by the diffusion methodology Agar and microdilution plates. In the in vivo test, the EEP 30% (w/v) was used as a sanitizer in handling before and after dipping in dairy cattle compared to the handling with iodine solution at 0.2% concentration, at 35 days. From the results found in the minimum inhibitory concentration test (MIC), you can say that the EEP has inhibitory activity in 100% of tested bacteria at a concentration of 10% (w/v). The main bacteria isolated from the surface of ceilings and animal milk were Staphylococcus spp. Corynebacterium spp. and Streptococcus spp. The standard plate count of aerobic mesophilic/mL (SPC/mL) and the somatic cell count/ml (SCC/mL) in the experimental group with iodine showed no significant differences (P> 0.05) between baseline and end of treatment, while animals in the experimental group of propolis significant effect (P <0.05) on the SCC/ml compared to the initial end (176,000 and 98,000, respectively). Given these results, it is concluded that the EEP at a concentration of 10% (w/v), could be used as an alternative disinfectant for managing before and after dipping of cattle ceilings, as shown improvement in microbiological quality milk, in addition to having antimicrobial activity against major mastitis-causing bacteria, and beneficial to the prevention of this disease.