Relatório do estágio curricular supervisionado em medicina veterinária área de concentração: imunologia e biologia molecular

Abstract

This report aims to describe the activities carried out during the period of Supervised Curricular Internship in Veterinary Medicine (ECSMV), in concentration area of immunology and molecular biology, held on September 8 from 2022 to December 23, 2022, totaling 450 hours. ECSMV had guidance by professor Dr. Paula Fonseca Finger and supervision by professor Dr. Fabricio Rochedo Conceição, being carried out at the Applied Immunology Laboratory, located in Technological Development Center of the Federal University of Pelotas. At the During the period it was possible to follow the laboratory techniques aimed at use of recombinant proteins for use in veterinary vaccines against clostridiosis. Furthermore, it was possible to follow experimental activities animal, mainly with mice and rabbits. The report will describe the protein expression techniques, enzyme-linked immunosorbent assay (ELISA), SDS-Page Western blot, microbiology and animal experimentation activities. Still, it will be discussed about the potency test carried out for recombinant vaccines against animal clostridiosis, as well as the management of experimental animals.