Membranas bicamadas à base de quitosana e alginato de sódio como potencial tratamento em feridas cutâneas

Abstract

The development of materials based on chitosan and sodium alginate together with different types of antimicrobial agents has shown to be a potential material for application as a wound dressing. That said, the present work aims at the production of bilayer membranes of chitosan and sodium alginate (MBC) and bilayer membranes incorporating olive leaf extracts (BEM), aiming their application as skin dressings. Olive leaf extracts (EFO) were obtained by maceration using a Dubnoff metabolic bath at 88°C for 2 h, the values obtained for antioxidant activity and total phenolic compounds were 94,25 % and 114,49 mgGAE.g-1 . MBC presented the thickness of 0,14 mm, whilst the value obtained for MBE was 0,22 mm. It was also observed that the addition of the extract decreased the rupture strength of MBE membranes (2.99 MPa) in compared to MBC (4,21 MPa), while the percentage of elongation increased from 9,99 % for MBC to 14,69 % for MBE. However, the incorporation of EFO positively influenced the swelling index, fluid permeation and Moist Vapour Transmission Rate (MVTR) of the bilayer membranes as the values obtained for these parameters were 213,42 %, 2083,47 g.m-2 .day-1 and 2094,33 g.m-2 .day-1 , respectively. The addition of EFO in the polymer matrix did not cause a significant impact on the water vapor permeability results, as the values obtained were 1,46.10-11 g.m-1 . Pa-1 . s-1 for MBC and 2,81.10-11 g.m-1 . Pa-1 . s-1 for MBE. The FTIR spectra showed the presence of functional groups of phenolic compounds in the membranes, thus proving the presence of EFO in it. The thermogravimetric analysis showed that the greatest mass loss occurred in the range from 210 to 300 °C. The membranes developed in the present work showed inhibitory behaviors, confirmed by the inhibition halos at 12,19 mm against Escherichia coli. Regarding the obtained results, the membranes produced in the present study are promising for application as a wound dressing.