Extração e purificação de compostos bioativos da própolis marrom

Abstract

Propolis is composed of resinous, balsamic and gummy substances derived from plant from plant secretions, possessing several bioactive properties, of which, antimicrobial properties stand out. These properties may be linked to geographical and seasonal factors, resulting in seasonal factors, resulting in a great structural complexity of propolis. However, raw propolis is not ideal, as it may present undesirable impurities that are harmful to the consumer, requiring harmful to the consumer, requiring the application of techniques for the removal of impurities that enable the use of its properties, however, through purification techniques such as ultrafiltration, the compounds can still reach more satisfactory levels. In view of this, the present work aimed to to study various methods of extraction of bioactive compounds from propolis and the purification of its constituents of interest using ultrafiltration. A methodology used was the comparison of aqueous extracts obtained by metabolic bath and ultrasound techniques, characterization of the antimicrobial properties, phenolic compounds antimicrobial properties, total phenolic compounds and total antioxidant activity, as well as the application of ultrafiltration and precipitation for purification. The extracts obtained through metabolic bath at 70°C for 60 minutes obtained the best results with mg 6.28 GAE.g-1 BS for total phenolic compounds and 30.65% for total antioxidant activity, in addition to being able to inhibit the bacteria Staphylococcus aureus, but was not able to inhibit Escherichia coli, while the extract obtained through ultrasound at room temperature for 45 minutes obtained mg 0.94 GAE. g-1 for total phenolic compounds and mg 4.42 for total antioxidant activity, however, it was not able to inhibit both microorganisms, so the extract from the metabolic bath was adopted for purification. As for purification through membranes, several nominal sizes were used (3kDa, 5kDa, 10kDa, 30kDa), and results were obtained for inhibition of microbial growth of gram-negative bacteria ranging from 25.2%, 62.4%, 34.9% and 45%, respectively, while the results of inhibition of growth of gram-positive bacteria ranged from 33.8%, 0%, 54.1% and 63.9%, respectively. Precipitation was used in saturation ranges of 20%, 50% and 80%, with results of inhibition of microbial growth for gram negative bacteria ranging from 14.43%, 45.12% and 58.87%, however for the results referring to the inhibition of microbial growth of gram positive bacteria for the saturation ranges of 20%, 50% and 80%, results ranging from 32.60%, 43.76% and 40.43% were obtained.