Efeito protetor da farinha de banana verde (Musa sinensis) frente ao dano oxidativo induzido por ferro em Drosophila Melanogaster

Abstract

Many factors can contribute to metabolic dysfunction in the body. Exposure to chemicals, drugs, poor diet, and drug misuse are all factors that contribute to homeostatic dysregulation. Iron (Fe) is an essential micronutrient in biological processes in living beings. Children and pregnant women lack this mineral for the healthy development of the body, and generally, when there is a need, this replacement is done through food supplements. However, some individuals have dysregulation in the absorption of iron, something extremely harmful to cells, which can lead to several serious pathologies. For some years, the use of natural products has been investigated for the benefit of human health. Several foods have proven to be effective promoters of well-being. Functional products have gained prominence in recent times by the world population. The search for good physical shape and improved quality of life has promoted the use of these products. The aim of this study was to evaluate whether the green banana flour Musa sinensis (M. sinensis) has a reducing action capable of protecting against oxidative stress caused by acute food exposure to Fe in Drosophila melanogaster. The flies were divided into six groups: (1) control (8 days of agar feeding); (2) Fe (4 days of feeding on agar and after 4 days of exposure to 10 mmol ferrous sulfate); (3) FB10 (4 days of feeding with 10 mg/mL of green banana flour and after 4 days of feeding with agar); (4) FB50 (4 days of feeding with 50 mg/mL of green banana flour and after 4 days of feeding with agar; (5) CB10 Fe (4 days of feeding with 10 mg/mL of green banana flour and after 4 days ferrous sulfate 10 mmol); (6) FB50 Fe (4 days of feeding with 50 mg/mL green banana flour and after 4 days ferrous sulfate 10 mmol). behaviors (negative geotaxis and open field) and ex vivo, the activity of the enzyme acetylcholinesterase (AChE), determination of dopamine and octopamine levels, catalase activity (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) were analyzed. ), levels of lipid peroxidation (TBARS) and levels of reactive species (RS).Exposure to Fe significantly increased fly mortality, while survivors showed decreased climbing activity and hyperactive behavior in the open field test, with activity increased AChE, dopamine and octopamine. However, dietary supplementation of green banana flour showed improvement in locomotor activity and restoration of AChE activity. Feeding also prevented the increase in dopamine and octopamine and attenuated locomotor damage in flies exposed to the metal. Green banana flour showed antioxidant potential and mitigated the effect caused by acute exposure to Fe in D. melanogaster.