Membranas curativas com princípio ativo de folhas de oliveira e potencial para aplicações tópicas

Abstract

This work aimed to obtain biopolymeric membranes from materials of natural origin, by envi ronmentally friendly processes, for application as topical dressings in wound treatment. Chi tosan (polymer of natural origin) was used as matrix, natural extracts of olive leaves as additives and glycerol as plasticizer. Thus, pure chitosan membranes were prepared and added with olive leaf extracts, using the casting methodology. The membranes were characterized in terms of their morphological (SEM), physical (thickness), mechanical (tensile strength and elongation), barrier (TVW and WVP), fluid absorption (solubility and swelling) and inhibition and barrier properties against pathogenic microorganisms. The results for the pure and additive chitosan membranes with extract, respectively, were: thicknesses of 0.032 ± 0.009 mm and 0.040 ± 0.017 mm, tensile strength of 5.77 ± 1.35 MPa and 3,11 ± 0.71 MPa, elongation at break of 30.89 ± 4.30% and 31.53 ± 1.67%, TVA of 510.39 ± 12.10 g.m-2.day-1 and 581.84 ± 6.03 g.m -2.day-1, WVP of 6,23 ± 0,97 g.mm.kPa-1.day-1.m-2 and 5.52 ± 1.10 g.mm.kPa-1.day-1 .m-2, swell ing of 242.60 ± 12.41 % and 59.45 ± 3,60 %, solubility of 18,57 ± 1.64 % and 31.55 ± 5.79 %. The results of inhibition to the microorganism showed that the composition of pure chitosan did not present any effectiveness, while the composition added with extract of olive leaves showed good inhibition against the growth of E. coli and S. aureus. Both compositions showed good barrier properties in microbial permeation tests. The results found for these properties indicated that, in general, the membranes presented properties that allow their topical applica tion in the treatment of wounds, providing physical protection against mechanical wear, main taining a favorable environment for healing, inhibiting infections by microorganisms and stim ulating its recovery, with the additive membranes with olive leaf extract being highlighted mainly in the inhibition of microorganisms.