Ação protetora da Hesperidina nos danos hepático, renal e reprodutivo induzidos pela Zearalenona em camundongos

Abstract

The mycotoxins are secondary metabolites of fungi that have toxic effects in humans and animals. The zearalenone (ZEA) is produced by species of fungi Fusarium, and its occurrence is frequent in grains and cereals in all the world.The ZEA, the mycotoxin known to cause the oestrogenism in pigs, is a estrogenic mycotoxin having the reproductive system with addition of hepatic and renal system as one of its principal targets of toxicity. Already Hesperidin (HESP), a glycoside flavonoid found in citrus fruits, performs important beneficial activities because of its antioxidant property. Therefore, it is believed that HESP promote action against the toxic effects caused by ZEA. Were utilized male Swiss mices, divided into 4 groups (n=5): group 1 (Saline+ olive oil), group 2 (HESP 50 mg/Kg + olive oil), group 3 (Saline + ZEA 40 mg/Kg) and group 4 (HESP 50 mg/Kg + ZEA 40 mg/Kg).For 10 consecutive days were administered HESP (50 mg/Kg/day, orally) and from the 11th day was only administered to ZEA (40 mg/Kg/day, orally). After 48 hours, proceeded to euthanizing the animals, where blood and epididymis were removed. Were analyzed biochemical parameters: Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT), Alkaline Phosphatase (ALP), Gamma Glutamyl Transferase (GGT), Lactate Dehydrogenase (LDH), Urea, Creatinine, total bilirubin and fractions in addition to sperm parameters: count and sperm motility. The ZEA caused changes in all liver and kidney enzymes analyzed, as promoted reproductive changes with decreasing count and motility of spermatozoa. The pretreatment with HESP prevented the appearance appearing the toxic effects induced by ZEA in all parameters analyzed and so, this antioxidant can be used as an alternative therapy in the prevention of damage caused by ZEA.