Desenvolvimento de método de extração em fase sólida para compostos fenólicos em matriz aquosa com diferentes sorbentes

Abstract

Solid phase extraction (EFS or SPE - in English, Solid Phase Extraction) is a method of sample preparation that aims to clean, concentrate and extract the analytes of interest. In order to detect and quantify target substances such as phenolic compounds, solid phase extraction has been applied in several matrices, such as plant extract in water. In this perspective, this work aims to compare three SPE cartridges in the development of an extraction method that is capable of retaining nine phenolic compounds. An infusion simulation was used as a sample gallic acid, benzoic acid, caffeic acid, rutin, vanillic acid, siringic acid, cumáric acid, ferulic acid and quercetin, which were percolated by Strata C18- E, Strata X cartridges (Phenomenex, United States) and InertSep C18 (GL Sciences, Japan). Strata-X showed the best recoveries, retaining eight of the nine compounds and all with percentages above 77%. The Strata C18-E cartridge recovered only four compounds above 70% and the InertSept C18 cartridge showed the lowest recoveries, presenting only three compounds with values above 70%. The developed solid-phase extraction method proved to be effective and the sorbent that presented the best performance among the three fillings was Strata-X, presenting a greater amount of detectable compounds and significant recovery values.