Relatório do estágio curricular supervisionado em medicina veterinária - área de epidemiologia e saúde pública

Abstract

This report describes the activities developed during the Internship Supervised Curriculum in Veterinary Medicine in the research laboratory in leishmaniasis (LPL), leishmania collection from the Oswaldo Cruz Institute (CLIOC) and laboratory of national reference for leishmania typing (LRNTL), belonging to the Oswaldo Institute Cruz (IOC) of the Oswaldo Cruz Foundation (FIOCRUZ) in the municipality of Rio de Janeiro, in the epidemiology and public health. Most activities were linked to the project called “Verification of the expression of exhaustion markers and splenic disorganization in dogs with naturally infected visceral leishmaniasis ”. The activities performed involved training in quality and biosafety in a research laboratory, training isolation of peripheral blood cells and monitoring the laboratory routine, such as follow-up of autopsies of dogs diagnosed with visceral leishmaniasis, participation in the collection of clinical samples sent to the different methods of diagnosis and for research work, preparation of slides containing tissue cuts frozen spleen from infected dogs, histopathological evaluation of tissue sections of spleen from infected dogs, separation of peripheral blood mononuclear cells followed by freezing for future analysis, participation in weekly scientific seminars produced laboratory and analysis of mRNA expression by quantitative PCR, under the supervision of veterinarian and public health technologist at Fundação Oswaldo Cruz, Dra. Fernanda Nazaré Morgado and guidance from Prof. Dr. Débora da Cruz Payão Pellegrini. The period of The internship took place between September 16, 2015 and December 7, 2015, totaling a total of 450 hours.