Análise transcriptomica de prasiola crispa (lightfoot) kützing revela estratégias de resistência ao ambiente antártico

Abstract

The Antarctic continent is approximately centralized at the south of the Earth, being completely isolated in the meeting of the Atlantic, Pacific and Indic oceans. The Antarctic is a continent with the most severe conditions, where are present intense cold, physiological drought, severe winds and nutrient limitations. The composition of life present at Antarctic is very specific, with a presence of organisms including prokaryotes and eukaryotes, such as fungi, protists and algae. Among the algae, Prasiola crispa is an organism most commonly found, representing an important primary producer. However, the molecular mechanisms involved with the adaptive potential of P. crispa for this environment are still unknown. Therefore, to better understand the capacity of adaptation and development of P. crispa to these conditions, was made by Illumina-Hiseq 2500 sequencer, the achievement of the transcriptome this organism. Posteriorly, based on utilization of specific softwares, was made the respective assembly and annotation of the datas coming of sequencing. The assembly of the transcriptome of P. crispa resulted in 37,679 contigs, with an N50 of 1,389. The annotation procedure allowed the identification of the organisms with greater similarity with P. crispa, where stand out other Chlorophyta belonging to Antarctic environment. Can be performed the classification of the transcripts in three different categories, biological process, molecular functions and cellular components, as well as the view of their respective E-values. Lastly, can be performed the identification of 27 transcripts that possible help P. crispa to survive in the Antarctic environment. Being the same distributed in characteristics related to resistance and tolerance to low temperatures, high incidences of ultraviolet radiation, substances and nutrients transports and high salinity levels.