Estudo da Evolução da Protease 3c de Picornaviridae e Vírus Picorna-like Através da sua Sequência Proteica e Domínios Conservados

Abstract

The bees have a combination of individual features and animal cooperation is not yet found in the rest of the animal kingdom . They are social insects and participate in the pollination of many plants that provide food for man . In Brazil with the africanization of bees , these have become highly producing and swarm , which is making the country a power in the production of honey and other products derived from beekeeping . Many diseases can affect bees , among them many caused by viruses . Control viral infections is essential for ecological maintenance of bees and beekeeping . At the same time , it is possible to relate viruses that infect humans and viruses that infect the bees , whereas the treatments for humans could be used in beehives and at the same time using the bees as a model for development of new antiviral agents. In the search for a common point analyzed phylogenetically 3C protease , which occurs in the superfamily Picorna -like, which are viruses that parasitize bees virus. This protease is capable of cleaving the polyprotein, the mature viral proteins and viruses also cause the proteolytic degradation of host proteins . Until today there a way to use the 3C protease was found in phylogenetic studies because there is much divergence of their sequences between virus.The objective of this research was to identify a way to analyze phylogenetically 3C protease . The 3C protease and RdRp sequences of 55 viruses were collected from the National Center for Biotechnology Information , submitted to MEME , where four conserved sites were obtained . Upon phylogenetic analysis of the conserved sites and by maximum likelihood analysis of the distance between sites by parsimony was performed and was still a tree constructed based on the RdRp . Trees of sites 1 and 2 had a better statistical robustness and clustering of virus. These conserved regions of the 3C protease - Pro may be the start to establish a relationship between the proteases of the picornavirus and Picorna-like viruses in the quest to understand the mechanism of viral infection and also an alternative study for other sequences with high variability . The use of domains 1 and 2 provided the tree with greater robustness displayed until today for this viral protein.