Efeito anti-inflamatório da arnica montana em lesões cutâneas induzidas por radiação UVB em camundongos

Abstract

Ultraviolet radiation types A and B (UV) (315-400nm and 280-315nm respectively) are the main components present in sunlight known to cause damage and skin lesions. Arnica montana is a plant that has been widely studied for containing anti-inflammatory, healing and analgesic properties capable of preventing or ameliorating lesions. Therefore, the present study sought to investigate the therapeutic effect of topical application of Arnica montana (30 grams containing 30% Arnica montana dye) on parameters such as edema, oxidative stress and inflammatory reaction in the ear of mice after acute exposure to UVB radiation. The results of the present study showed that topical treatment with Arnica montana reduced the UVB-induced inflammatory response as demonstrated by the reduction of ear edema and the inhibition of myeloperoxidase activation and the gene transcription factor of inflammatory molecules (nuclear fator kappa B, NF-κB), which contributed to the reduction of inflammatory cytokines such as interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ). In addition, Arnica montana treatment ameliorated oxidative damage mediated by UVB radiation, as demonstrated by the reduction of lipid peroxidation, protein oxidation and elevated tissue antioxidant and glutathione content. In conclusion, the present study demonstrated the efficacy of homeopathic treatment with Arnica montana ointment in alleviating the inflammatory process and oxidative damage induced by acute UVB radiation, sustaining the traditional use of Arnica montana for the treatment of skin disorders.