Avaliação comportamental e bioquímica em Drosophila melanogaster expostas ao composto selenonucleosídeo derivado de zidovudina

Abstract

To assess the effects of zidovudine-derived selenonucleoside compound by in vivo and ex vivo assays on Drosophila melanogaster in order to verify possible pharmacological or toxicological effects as well as to verify the concentration at which the compound exhibits biological activity. Drosophila melanogaster of both sexes, adult, with a maximum of 3 days of age were divided into five groups of 30 flies each, being: (1) control, (2) 1 μM, (3) 10 μM, (4 ) 25 μM and (5) 50 μM of the selenon nucleoside compound added to 1% aqueous sucrose solution in filter paperto serve as food for the flies. The acute treatment had a total time of 48 hours, and the open field behavior and negative geotaxia tests were performed every 24 hours as well as the live fly count for mortality evaluation. At the end of 48 hours the flies were euthanized and prepared for the biochemical analyzes of lipid peroxidation and activity of the antioxidant enzymes catalase, superoxide dismutase and acetylcholinesterase. The results did not present significant differences in the tests of survival and locomotive of open field and geotaxia negative, demonstrating that the studied concentrations of the compound do not present toxicity in model of Drosophila melanogaster. In the ex vivo assays of lipid peroxidation and activity of the antioxidant enzymes catalase, superoxide dismutase and acetylcholinesterase, there were also no changes, their levels remained stable in all groups studied. The compound selenonucleoside did not present toxic effects in the evaluated parameters, being safe for the use in future studies in this model.