Avaliação de parâmetros bioquímicos, nutricionais e inflamatórios de camundongos velhos pré-tratados com óleo de Baru (Dipteryx alata)

Abstract

The changing age structure of the population is increasing in Brazil. Due to the higher number of elderly people, it is necessary to find alternative ways to prevent and control diseases. Among the alternative therapies, there are the natural with fewer side effects and drug interactions. The Baru (Dipteryx alata) is an abundant occurrence of fruit in the Brazilian cerrado. Isolated reports based on home medicine suggest that the oil extracted from the almond has important benefits, particularly antioxidant, anti inflammatory and antirreumática function. These characteristics of the feed transfer to the same great potential to use it in the food and pharmaceutical industry. Thus, the project has aimed to determine whether pretreatment with Baru oil will result in beneficial changes in old mice. Swiss mice used were young (3 months old) and old (20 months old), weighing 30-35 g, kept under light / dark cycle (12 hours) and temperature (22 ± 2 ° C). The animals are divided into 4 groups, wherein the oil Baru at doses of 20 mg / kg and 100 mg / kg and vehicle (canola oil - 10 ml / kg) were administered orally by gavage for 30 consecutive days . On the 31st day the animals receive a dose of pentobarbital (180 mg / kg, intraperitoneally) and blood is collected by cardiac puncture. They will be analyzed biochemical parameters (glucose, insulin, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides and uric acid), inflammatory parameters (rheumatoid factor (RF), protein C-reactive protein (CRP) and streptolysin-O (ASO)) and nutritional parameters (body weight). Statistical analysis will be performed by GraphPad Prism 5.0 through variance analysis (ANOVA) of a path followed by Newman-Keuls test, and the level of significance p <0.05. It was found that pretreatment with Baru oil promoted benefits to mice via modulation of the biochemical parameters analyzed inflammatory.