Efeito da suplementação da ração com o fungo pleurotus citrinopileatus singer na capacidade de reprodução de drosophila melanogaster (meigen, 1830)

Abstract

The species of fly, Drosophila melanogaster (Meigen, 1830) is a reference in biological and genetic studies, highlighting to be easily maintained in the laboratory and especially for having metabolic reactions similar to mammals. In nature uses as food yeasts that colonize fruits, flowers and fungi in stage of putrefaction. The mushrooms of the genus Pleurotus are widely used in human, rich in proteins and antioxidants power when added to animal feed increases its nutritional value. Fungi of this genus are noted for easily grow in a wide variety of substrates which facilitates its cultivation and extends its biotechnological use. This paper aims to identify the effect of Pleurotus citrinopileatus the organism Drosophila melanogaster. First mycelium of P. citrinipileatus developed on PDA (potato-dextrosee-agar) was homogenized ration consisting of corn flour (Klein et al, 1999) and provided as food for D. melanogaster, these were kept in BOD at 23 ° C ± 1. After the flies were removed from contact with the fungus and kept without substrate mycelium. The descendants of flies that were fed aviam P. citrinopileatus, but have not received the diet with homogenized mycelia were observed up to the third generation to identify the effects of dietary supplementation with fungus would be homogeneous in subsequent generations. Finally, we tested different concentrations of the substrate mycelium. The results were tested by analysis of variance with the help of Statistix8 program. It was observed that the mycelium of P. citrinopileatus stimulated reproduction in D. melanogaster, significantly increasing the number of individuals per reproductive cycle. The concentration of 0.12 g mycelium / g substrate was best expressed this feature. The stimulation occurred only when exposed to substrate mycelium, not permanent and not transmitted to subsequent generations. The experiment suggests that the use of mycelium of P. citrinopileatus added to the ration D. melanogaster cultured in the laboratory, can be used as a reproductive stimulant.