Bloqueio neuromuscular induzido pela peçonha de bothriurus bonariensis sobre a preparação nervo músculo de phoetalia pallida

Abstract

he species Bothriurus bonariensis, popularly known as the black scorpion, though not often induce severe clinical conditions is easily found in the urban environment. Venoms animal have been widely recognized as a major source of biologically active molecules. Given the broad spectrum of use of these active biomolecules found in venom scorpion, to characterize the protein fraction of the venom becomes interesting not only terms the standpoint of biotechnology, but also ecological, because then we can better understand this scorpion. The scorpions were collected using pitfall traps and falling scattered on campus and in the town of Cerro Batovi, São Gabriel, RS. The venom was extracted from individuals by various electrical stimulation directly to the last abdominal segment and immediately transferred to 0.9% saline solution. The animals were anesthetized by freezing at -5 ° C, and posted on the dissection plate. After dissection of the cuticle, the metathoracic region, the nerve was exposed and 5 electrodes Ag/AgCl inserted for electrical stimulation. The recordings were made using isometric software AQCAD for 120 min. The analyzes were performed by software ANCAD. The different treatments with the venom (0,4μg/μl, 0,8μg/μl, 1,6μg/μl e 3,2μg/μl) were made by injection in the abdominal portion of the third region with the aid of a Hamilton syringe. With our results, we observed a dose- dependent on the strength of muscle contraction. When the dose ( 8ug/g animal) was tested , there was a 50% inhibition of the contractile response by 60 ± 2 minutes , and 55 % inhibition at the end of 120 minutes of recording (n = 5 , P <0.05 ) . None of them concentrations tested to induced an increase in the contractile response in the presence of venom. The results suggest that the venom of B. bonariensis induced peripheral neurotoxicity when administered to cockroaches. Furthermore work is needed to make better characterize this venom and so we can answer what fraction of the venom that has activity and the mechanism of action.