Avaliação do Efeito Gastroprotetor da Cutícula Coilina Retirada do Ventrículo de Gallus Gallus Domesticus em Ratos

Abstract

Gastric ulcer is considered one of the major gastrointestinal disorders usually with chronic evolution. Conventional therapy is performed by concomitant use of antibiotics and drugs-inhibition of acid secretion, to enhancing the rapid healing of gastric mucosa. The popular knowledge is the main determinant in the use of biological materials by population, in western of Rio Grande do Sul/Brazil, there is a popular culture of the use of coilin cuticle (C) of chicken gizzard as natural substance for the treatment of gastric disorders. No scientific reports were found proving its gastroprotective effect, so the objective of this study was to determine the activity and possible gastroprotector mechanisms of C in organic acids induced ulcer model by acute treatment with the prospect of a new therapeutic alternative. Therefore, the C was donated clean by farmers, dried and ground for use of experimental protocols. It were used female Wistar rats, 3 months, n = 5/group. The gastric injuries were caused with absolute ethanol (cooled; 1mL/200 mg) and acetylsalicylic acid (AAS; 400mg/200mg), evaluated by macroscopic and microscopic visualization and stomacher tissue were submitted to histology with hematoxylin-eosin. To start, it was investigated the possible mechanisms by quantifying mucus stuck in the stomach mucosa and gastric acidity (acid titration) and its volume. At the same time, it was determinate the H + ,K + -ATPase activity in the microsomal fraction of the gastric mucosa by spectrophotometry. The results were expressed in MSEM and 1-way ANOVA with post-hoc Tukey's were used, p <0.05 was considered significant. The results obtained showed that C was able to protect the gastric mucosa of the injuries-induced, in the concentration of 5 mg/Kg, with cytoprotective action. Also, the C increased the production of gastric mucus in both ulcerative investigations; increase the pH measurement of basal form and inhibited H + ,K + -ATPase specific activity. Thought, the coilina, used by Rio Grande Sul community, showed very promisor results in gastroprotective action.