Análise de estresse oxidativo em células espermáticas congeladas de bovinos com a adição de antioxidantes naturais

Abstract

The importance of this project’s achievement is based on the bovine culture appreciation in the market because the semen samples need to reach the final destiny less damaged as possible to be successful used in artificial insemination (AI). Therefore, in the cryopreservation process is necessary to use diluents which protect the spermatic cells. This projects aims to test different plant’s extracts as antioxidants agents during cryopreservation in bovine semen. In addition, the evaluation of (a) seminal patterns after thawing in the presence of different antioxidants, (b) the effects of these antioxidants on the motility and sperm vigour, (c) measuring cell damage using TBARS assay and CAT assay. The sêmen collection started the procedure followed by the preliminary volume, aspect, vortex, motility and vigour analysis under microscope. Furthermore, the samples are separated in six different groups: positive control (20% egg yolk), negative control (basic medium) and groups treated with antioxidants (Anacardium microcarpum, Stryphnodendron adstringens, Croton campestris e Duguetia furfuraceae), all the sample were also treated with 6% glycerol, followed by three steps: 3h (4°C), 20 min at nitrogen liquid vapour (N2) (-70°C) and finally N2 immersion. Posteriorly, the samples were thawed and analysed with the same previous parameters, besides the biochemical assays TBARS and CAT activity for evaluation of the sperm samples during the cryopreservation process. The results showed the plant’s extracts as viable alternative, but more studies are needed to identify which extracts are truly acting as cell protector during the freezing process.