Bioprospecção de micro-organismo com potencial para produção da enzima Tanase

Abstract

The tannase is an enzyme characterized by the hydrolysis of esters and lateral connections glucose hydrolysable tannins and gallic acid, is found in a range of plant species as well as in the mucosa of the ruminant intestines of cattle, however, the main producers and most studies focus on micro-organisms, such as filamentous fungi, yeasts and bacteria. This enzyme has many applications such as production of gallic acid, used in the pharmaceutical industry, production of instant teas, clarification of juices, beers and wine processing, pre-treatment of animal feed, in the treatment of effluents from tannery and leather industry . Despite numerous applications of tannase production is still considered a process of high manufacturing cost, by going through a long period of biotechnological processes and the lack of bodies that enable greater production. Knowledge of biodiversity and bioprospecting of new bodies have become a major focus of the biotechnology era, since the use of these organisms in the search for solutions in several areas such as in the food, health, environment and industry is growing at an accelerated rate in view of this, the objective of the project is the micro-organisms with potential for production of tannase from natural plant sources bioprospecting. Organisms with potential production of tannase - bioprospecting of plant sources such as bark acacia, banana, rice bran and tails, leaf yerba mate and quince for isolating micro was performed. These samples were cleaned and incubated at 25 ° C on potato agar for four days after the microorganisms have been isolated that have developed specific Cezapek Dox medium and incubated bodies which showed the formation of inhibitory halo are identified as producers of tannase . Seven manufacturers of tannase fungi were identified, demonstrating the viability of plant sources for bioprospecting of tannase-producing.