Investigação de alterações comportamentais e oxidativas induzidas por suplementação com gordura interesterificada e posterior exposição ao ferro em D. melanogaster

Abstract

The change in eating habits has significantly contributed to the increase in the consumption of ultra-processed fats. Among the most commonly used synthetic fats in food products is Interesterified Fat (IF). However, some studies have already shown that the intake of this fat is harmful to health. Therefore, the objective of this study was to evaluate whether acute exposure to a low concentration of iron (Fe) affects the behavioral and oxidative parameters in D. melanogaster flies previously exposed to IF. The flies were divided into four experimental groups: I) Control; II) IF 5%; III) Fe 5 mM; IV) IF 5% + Fe 5 mM. First, they were exposed to IF for 7 days, and then they were exposed to an iron solution for 24 hours. Subsequently, they underwent behavioral tests for locomotor activity (open field maze and negative geotaxis). After the behavioral analyses, they were euthanized and homogenized for oxidative state analyses (reactive species and resazurin reduction). In this way, we found that flies exposed to IF and iron showed greater damage (motor and oxidative).