Análise e avaliação qualitativa da expressão de transcritos que Codificam proteínas de membrana a partir da determinação de Limiar de presença/ausência

Abstract

The development of highthroughput techniques in the field of molecular biology brought the born? of genomics. Since then the generation of biological data rose in a exponential way and a good share of these data are freely available in biological databases like NCBI. The microarray technique analyses the mRNA transcripts in a global approach and for a long time the most popular technique in the field of functional genomics. The microarray raw data are created trough the scanning of light signals intensities produced by the hybridization of transcripts with its probes targets. It is necessary a preprocessing step to convert the light signal to expression values. Several preprocessing algorithms was developed but none is absolute, due to different results produced depending on which preprocessing method was choose. Few methods to determine the presence/absent of genes was created for microarray. Those whom was developed have some limitation concerning data exploration on different microarray platforms or the method of preprocessing. In the present work, a new method of detection call which permits the use of any preprocessing method and microarray platform was developed based on empirical data of flow cytometry using human cells of the immunological system.