Caracterização de SNPS putativos entre eugenia uniflora e Eucalyptus Grandis Hill ex Maiden.

Abstract

Eugenia uniflora (Surinam cherry), is a species of the Myrtaceae family, quite economically exploited due to their pharmacological properties. Therefore, studies at the molecular level on this species are important. SNPs have been a widely used tool in the area of genomics, as well as ESTs also offer several advantages due to its presence in various data banks. The aim of this study was to search for SNPs in gene regions of E. uniflora co mpared to the ESTs database of E. grandis. For this study, total DNA of E. uniflora was sequenced, generating a coveragem of about 25% of the species genome, in about 2,600 contigs. Out of these, 150 were selected to search for hypothetical gene regions, by comparing to the EST database of Eucalyptus grandis. Only contigs containing minimum coverage 80pb were selected. Out of the 150 contigs analyzed, 35 ESTs were obtained with similarity between the two species. After the removal of introns, some contigs showed fragmented alignments and others showed a lower coverage 80pb. Of the total analyzed, 8 contigs met all the required qualities. The contig that showed a lower frequency (SNPs / pb) showed similarity to the sequence that expresses the Eto1 protein. The contig that presented a intermediary value regarding the maximum and minimum frequency revealed similarity with the RPK protein, while the contig that presented higher SNPs freqeuncy showed similarity with the AGP protein. The number of transitions was higher than the tranversions. These data were classified as low, and indicate that these gene regions are well conserved. The technique is easy to use and very useful even if it is little used.