Desinfestação de segmentos nodais de eugenia involucrata dc. através de nanopartículas de ouro e de prata visando à propagação in vitro

Abstract

In vitro propagation or micropropagation is one of the techniques of Plant Tissue Culture that aims at the large scale cultivation of plants free of contaminants. These may be bacterial or fungal in nature, which in excess can lead the explant to death. New methodologies arise to improve this technique, and one innovation is the use of nanoparticles in disinfestation of explants. Eugenia involucrata DC. known as cherry of the Rio Grande belongs to the family Myrtaceae that has 130 genera with 4000 species. Because it has resistant wood and economic potential to use of its fruits, it is important to cultivate this species, which is also used in the recovery of degraded areas. The present work aimed to use silver nanoparticles (AgNps) and gold nanoparticles (AuNps) in the disinfestation of Eugenia involucrata DC. compared to other different treatments. In the experiment, five types of treatment were used: (1) control composed of sodium hypochlorite and 70% alcohol, (2) dichloro, (3) antibiotic, (4) silver nanoparticles and (5) gold nanoparticles. The results showed that the silver nanoparticles offer a more effective disinfestation in comparison to the other treatments for this species.