Análise do polimorfismo p72r do gene tp53 e sua possível relação com a gemelaridae em Cândido Godói, RS.

Abstract

Cândido Godoi, located in the northwest of Rio Grande do Sul state, has a high note of twin births, serving as a source for the studies that seeking to understand the mechanisms involved in this phenomenon. The aim of this study was to compare the frequency of polymorphism P72R of TP53 gene in women with and without twin births historical to, if possible, correlate with the twinning phenomenon in Cândido Godói. The DNA was extracted from blood samples and the polymorphism was genotyped by real time PCR. Were genotyped 42 mothers of twins and 101 mothers of singletons. After the statistical analysis was observed a difference in the allele frequencies (p= 0,0006) and genotypic (p= 0,0007) among cases and controls. When the analysis was done controlling the women by the inbreeding coefficient (F), no statistical difference was obtained, but we see that there is a tendency for women with a higher value of F possess the CC genotype. As the C allele is considered a risk factor for failure of blastocyst implantation, the results obtained here were the opposite of the expected. Twin mothers have the C allele in higher frequency than the control mothers. An explanation for this discovery would be that, to be a transcription factor for many genes, this polymorphism may be affecting another gene in the p53 pathway, resulting in the “reversed” frequencies that we found in this study.