Utilização de gonatrofina coriônica equina e extrato bruto de carpa na indução a reprodução de jundiá (Rhamdia quelen)

Abstract

The most used technique for the production of dehydrated carp pituitary gland in the induction of human chorionic gonadotrophin gland (HCG) and gonadotropin releasing hormone analogues (GnRHa) are also widely used in the induction of an unblocking. the induction of spawning, Equine Chorionic Gonadotrophin (ECG) is widely used in the reproduction of viruses to act directly on the ovary, aiding in follicular growth. The objective of this work was to analyze the use of equine chorionic gonadotrophin (ECG) and crude extract of carpal hypophysis (EBHC) consortium or not in the induction of reproduction of Jundia (Rhamdia quelen). The experiment was carried out at the Federal University of Pampa, in the Laboratory of Fish and Aquaculture - LAPA in March 2019. Six females with a mean weight of 719.25 ± 175.17g and 12 males with 575.11 ± 71, 03g respectively, in a completely randomized design with three treatments and two replicates. The treatments were: I: Gross carp pituitary extract (EBHC) 3mgkg-1 (1st DOSE: 0.3 mgkg-1; 2nd DOSE: 2.7 mgkg-1); II: Gross carp pituitary extract (EBHC) + Equine chorionic gonadotrophin (ECG) (1st DOSE: 0.3 mg kg -1 of EBHC; 2nd DOSE: 2.7 mg kg -1 EBHC + 500 IU kg -1 of ECG); III: Equine chorionic gonadotrophin (ECG) (1st DOSE: 0.3 mg kg -1 of EBHC, 2nd DOSE: 500 IU kg -1 of ECG). The parameters analyzed were: Percentage of spawning efficiency; Fertilization rate; Accumulated thermal unit (U.T.A.); Ovocytes released; Oocytes produced; total spawning oocytes relative to hatched larvae. The results were submitted to analysis of variance (5%) and the means were compared by the Tukey test. The results obtained in the experiment presented significant differences (P <0.05) for fertilization rate and U.T.A. when induced with the hormones consortium or not. It is concluded that EBHC has been shown to be more efficient, but the ECG can replace it when the animals have reproductive characteristics in full development.