Produção de curativo biopolimérico bicamada composto de gelatina e eletrofiado com poliácido láctico

Abstract

The present study aimed to develop biopolymeric bilayer membranes by combining casting and electrospinning techniques. The methodology involved preparing 10% (w/v) gelatin films and depositing poly(lactic acid) (PLA) fibers onto them. Electrospinning was conducted with a flow rate of 0.8 mL/h, temperature of 23 ± 2 °C, relative humidity of 55 ± 2%, voltage of 20 kV, a 0.8 mm nozzle, and a distance of 18 cm between the collector and the nozzle. An experimental design was developed to assess the influence of PLA solution concentration and volume on the formation of electrospun fibers. The characterization of the produced bilayer membranes included thickness, water vapor permeability (WVP), solubility, and morphology analyses. In terms of results, the thickness ranged from 0.3 to 0.5 mm, WVP varied from 2.58x10-10 to 4.12x10-10 (g-1Pa-1s-1), and solubility showed a range of 73.71 to 81.63%. The experimental design indicated that the smallest thickness is achieved with lower fiber deposition on the membrane. The highest water vapor permeability is obtained with lower concentrations of the PLA solution, and solubility is not influenced by any of the analyzed factors. Thus, the condition deemed suitable for the development of biopolymeric bilayer membranes is a concentration of 3.77% and a deposition volume on the gelatin membrane of 1.93 mL, i.e., the optimized value in desirability. In conclusion, the combination of casting and electrospinning techniques for the production of a bilayer membrane demonstrates potential application as a topical wound dressing, proving to be a suitable approach.