Efeito do Resveratrol na criopreservação de sêmen de garanhões da Raça Crioula

Abstract

The possibility of transporting genetic material over long distances makes the use of frozen semen highly necessary for the equine industry, but the low fertility rates resulting from cellular damage by processing limit its use. The factors that because damages are diverse, among them, the production of reactive oxygen species (ROS) and oxidative stress stand out. The addition of antioxidants in the diluent can decrease damages, preserving sperm parameters and increasing the fertility of frozen semen. Resveratrol (RSV) is a polyphenol derived from plants and has some biological properties, including antioxidant effect in several systems. Thus, the aim of this study was to check the action of RSV added to the diluent for freezing the semen of stallions of the Crioula breed on sperm viability and oxidative stress. For this purpose, four Cioulos stallions, with known fertility, had semen collected three times during the breeding season. The semen free of gel fraction was diluted and fractionation in two aliquots, one frozen in Botucrio® diluent without any supplementation (control), and the other in diluent supplemented with 10 μM RSV. After maintaining the semen in liquid nitrogen, two straws from each group were thawed and subjected to evaluations of sperm kinetics, longevity, morphology, plasma membrane integrity, acrosomal and mitochondrial membrane potential, ROS production and total antioxidant potential. With this study, it we concluded that the addition of 10 μM of RSV in freezing diluent did not influence the kinetic, biochemical and functional parameters of thawed sperm from Crioulos stallions. Further studies should be carried out to define oxidative stress profiles and adequate concentrations to preserve the quality of thawed semen.