Efeito protetor da 7-cloro-4- (fenilselanil) quinolina em um modelo “tipo diabetes” induzido por dieta hiperglicídica em drosophila melanogaster

Abstract

Diet is a key component for the development and longevity of organisms. In this scenario, special attention has been given to carbohydrates, since sugar-rich diets have been linked to metabolic alterations such as obesity and insulin resistance, which are important characteristics of type 2 diabetes mellitus. The objective of this work was to use the fruit fly Drosophila melanogaster to evaluate the effects of hyperglycidic diet (HD) and to evaluate the protective effect of 7-chloro-4- (phenylselenyl) quinoline (4-PSQ). Adult flies were divided into eight groups of 50 flies each: (1) RD, (2) RD + 4-PSQ (25 μM), (3) regular diet (HD) 5%, (4) HD 10%, (5) HD 30% (6) HD 5% + 4-PSQ (25 μM), (7) HD 10% + 4-PSQ (25 μM) and (8) HD 30% + 4-PSQ (25 μM). Flies were exposed to a diet containing sucrose and / or 4-PSQ for 10 days, according to each group. At the end of treatment the survival rate, longevity, hatch rate, food intake, glucose and triglyceride levels, as well as some markers of oxidative stress, such as thiobarbituric acid reactive substances (TBARS ), enzyme activity, superoxide dismutase (SOD) and catalase (CAT), protein thiol (PSH) and non-protein levels (NPSH) and cell viability assays: Resazurin and MTT. It was observed that the consumption of DH diets was associated with lower survival of the fly, lower longevity and increased levels of glucose, triglycerides, TBARS and increased activities of enzymes, SOD and CAT. Treatment with 4- PSQ increased the satiety of flies, increased survival, reduced glucose, triglyceride and TBARS levels, increased hatching, as well as normalized enzyme activity, SOD and CAT. These experiments suggest that 4-PSQ had apotential antioxidant effect and provided greater satiety by attenuating the effects of high HD consumption on a diet-induced diabetes-like model in Drosophila melanogaster.