Efeito de n-acetilcisteína na desinfestação de eugenia uniflora l.

Abstract

The Surinam cherry (Eugenia uniflora L.) is an important fruit species belonging to the Myrtaceae featuring herbal and ornamental potential. One of the difficulties in the micropropagation of this kind is to obtain tissues free of contamination. In order to improve their multiplication in vitro experiment was conducted to verify the effect of N - acetylcysteine on disinfestation of Eugenia uniflora L. yolks Studies were conducted at the Laboratory of Plant Tissue Culture - UNIPAMPA, which developed two treatments, A (no N-acetylcysteine ) and B ( N- acetylcysteine ). The pest was held in the chapel of horizontal laminar flow. In treatment A yolks were dipped in 70% ethanol for 1 minute followed by immersion in a solution of sodium hypochlorite 1,25 % of active chlorine for 20 minutes with three rinses in autoclaved distilled water and then dipped in chloramphenicol for 2 hours. In treatment B gems were immersed in 3 % N - acetylcysteine for 6 hours , 70% ethanol for 1 minute followed by immersion in a solution of sodium hypochlorite 1,25 % of active chlorine for 20 minutes with three rinses in distilled water autoclaved and then dipped in chloramphenicol for 2 hours. The explants were cultured in test tubes containing MS medium with agar and sucrose and AIB 0,1 mg and 0.2 mg BAP. After the 9th day of culture was evaluated bacterial and fungal contamination. In the decontamination of the buds, the observed rate of bacterial contamination was 17,30% in treatment A and treatment B 40,38 % rate in treating fungal contamination was 42,31 % A and treatment B was 57,69%. After the 16th day of inoculation survival rates were observed in 13,46% in treatment A and 7,69% in treatment B.