Avaliação do efeito antileucêmico de amostras de própolis de 4 municípios do Rio Grande do Sul

Abstract

Propolis, "bee glue" and / or "black wax", is similar to natural wax resinous substance found in beehives. This substance is used by bees to seal cracks or open spaces, serves as an antiseptic and prevents microbial infection of larvae, holds moisture and controls the internal temperature of the hive. The composition of the crude propolis is basically divided into 50% from vegetable resin, 30% beeswax, 10% essential oils, 5% pollen, 5% scrap wood and soil. This natural compound has been used for various therapeutic purposes in humans and animals due to their pharmacological properties. Numerous scientific papers have demonstrated that propolis has bactericidal, bacteriostatic, antifungal, analgesic, healing, anti-inflammatory, antioxidant and antitumor activities. The chemical composition of propolis can be highly variable depending on geographic location, time of harvest technique and genetic Bee. The aim of the study was to evaluate the antitumor effect of different propolis samples of Rio Grande do Sul, using as a model cell line K562. Treated with different concentrations of propolis extract cells were evaluated for viability and cell cycle by flow cytometry at 24h, 48h and 72h. The results showed that the concentration of propolis analyzed 100μg / ml decreased from 24 hours of treatment and 48 hours of treatment all samples showed viability of some kind of change in the cell cycle. Furthermore, when comparing the results of cell viability of the state propolis with data from another study made with propolis, at the same concentration, propolis Rio Grande do Sul demonstrates greater antineoplastic effect, causing increased cell death and shorter treatment time. These preliminary results show that propolis from Novo Hamburgo, São Gabriel, Taquara and Vacaria could be an option in the search for new drugs for the treatment of leukemia, with more analysis in different neoplastic cell lines with propolis and other municipalities of the State of Rio Grande do Sul are being conducted by our research group.