Análise da população de bactérias ácido-láticas (bal) vaginais cultiváveis em resposta ao estrógeno e sua influência no potencial oxidante e antioxidante do ambiente vaginal em ovis aries

Abstract

Microbiota is the used term to characterize the community of microorganisms that inhabit tissues and biological fluids of multicellular organisms. Divergences in this composition in several cases may be related to the development of various infectious, hepatic, metabolic, respiratory, mental, autoimmune and intestinal neoplasms. This proposal aimed to characterize the fluctuations of the cultivable population of lactic acid bacteria (LAB) in the vaginal environment in response to estrogen in cut sheep. In addition, this work verified the correlation between the presence of cultured LABs in the vagina and the oxidative potential of this environment. To do so, experiments were carried out to characterize the vaginal microbiota and the oxidant/atioxidant potential of this environment in response to estrogen divided into three groups (n = 15): (1) controls (D- 2.5), (2) 12h after injection of estradiol benzoate (D.0.5) and (3) 60h after injection of estradiol benzoate (D.2.5). The results suggest a appropriate in vivo model for studying vaginal microbiota and oxidant/antioxidant environment which was able to detect effects of estradiol benzoate (EB) on the assessed variables. In conclusion, estrogen acutely regulates the vaginal environment by altering the population of vaginal LAB and the balance between oxidant and antioxidant activity. Lastly, particularities of the validated in vivo model warrant further investigation to address recent questions that raised from the data generated.